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Subcellular Duplex DNA and G‐Quadruplex Interaction Profiling of a Hexagonal PtII Metallacycle

Domarco, Olaya et al.

Angewandte Chemie. Volume 131:Number 24 (2019); pp 8091-8096 -- WILEY-VCH Verlag

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  • Title:
    Subcellular Duplex DNA and G‐Quadruplex Interaction Profiling of a Hexagonal PtII Metallacycle
  • Author: Domarco, Olaya;
    Kieler, Claudia;
    Pirker, Christine;
    Dinhof, Carina;
    Englinger, Bernhard;
    Reisecker, Johannes M.;
    Timelthaler, Gerald;
    García, Marcos D.;
    Peinador, Carlos;
    Keppler, Bernhard K.;
    Berger, Walter;
    Terenzi, Alessio
  • Found In: Angewandte Chemie. Volume 131:Number 24 (2019); pp 8091-8096
  • Journal Title: Angewandte Chemie
  • Subjects: Chemistry--Periodicals; G-Quadruplexe--Metallacyclen--Platin--SCC--Subzelluläre Lokalisierung; Dewey: 540
  • Rights: legaldeposit
  • Publication Details: WILEY-VCH Verlag
  • Abstract: Abstract:

    Metal‐driven self‐assembly afforded a multitude of fascinating supramolecular coordination complexes (SCCs) with applications as catalysts, host–guest, and stimuli‐responsive systems. However, the interest in the biological applications of SCCs is only starting to emerge and thorough characterization of their behavior in biological milieus is still lacking. Herein, we report on the synthesis and detailed in‐cell tracking of a Pt2L2metallacycle. We show that our hexagonal supramolecule accumulates in cancer cell nuclei, exerting a distinctive blue fluorescence staining of chromatin resistant to UV photobleaching selectively in nucleolar G4‐rich regions. SCC co‐localizes with epitopes of the quadruplex‐specific antibody BG4 and replaces other well‐known G4 stabilizers. Moreover, the photophysical changes accompanying the metallacycle binding to G4s in solution (fluorescence quenching, absorption enhancement) also take place intracellularly, allowing its subcellular interaction tracking.

    Abstract :

    Biologische Anwendungen supramolekularer Komplexe: Ein hexagonaler cyclischer Platinkomplex akkumuliert im Nucleus von Krebszellen und führt zu einer spezifischen blauen Fluoreszenzfärbung von Chromatin. Dank der einzigartigen photophysikalischen Eigenschaften des Komplexes konnte seine subzelluläre Akkumulation in G4‐reichen Nucleoli aufgedeckt werden.

  • Identifier: System Number: LDEAvdc_100085529657.0x000001; Journal ISSN: 0044-8249; 10.1002/ange.201900934
  • Publication Date: 2019
  • Physical Description: Electronic
  • Shelfmark(s): ELD Digital store

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