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Polarized Raman Anisotropic Response of Collagen in Tendon: Towards 3D Orientation Mapping of Collagen in Tissues

PLoS One, May 2013, Vol.8(5), p.e63518 [Peer Reviewed Journal]

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  • Title:
    Polarized Raman Anisotropic Response of Collagen in Tendon: Towards 3D Orientation Mapping of Collagen in Tissues
  • Author: Galvis, Leonardo ; Dunlop, John ; Duda, Georg ; Fratzl, Peter ; Masic, Admir
  • Found In: PLoS One, May 2013, Vol.8(5), p.e63518 [Peer Reviewed Journal]
  • Subjects: Germany ; Crystal Structure ; Orientation ; Luminous Intensity ; Anisotropy ; Molecular Structure ; Bones ; Biology ; Polarization ; Biomedical Materials ; Peptides ; Polarization ; Helices ; Collagen ; Tissues ; Lasers ; Incident Light ; Collagen ; Microscopy ; Spectroscopy
  • Language: English
  • Description: In this study, polarized Raman spectroscopy (PRS) was used to characterize the anisotropic response of the amide I band of collagen as a basis for evaluating three-dimensional collagen fibril orientation in tissues. Firstly, the response was investigated theoretically by applying classical Raman theory to collagen-like peptide crystal structures. The theoretical methodology was then tested experimentally, by measuring amide I intensity anisotropy in rat tail as a function of the orientation of the incident laser polarization. For the theoretical study, several collagen-like triple-helical peptide crystal structures obtained from the Protein Data Bank were rotated “in plane” and “out of plane” to evaluate the role of molecular orientation on the intensity of the amide I band. Collagen-like peptides exhibit a sinusoidal anisotropic response when rotated “in plane” with respect to the polarized incident laser. Maximal intensity was obtained when the polarization of the incident light is perpendicular...
  • Identifier: E-ISSN: 19326203 ; DOI: 10.1371/journal.pone.0063518

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